Davidson's fixative ，戴维森氏固定液，davidson固定液2
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Davidson's Fixative/Hartmann's fixative
Hartman固定剂，也称为Davidson固定剂，含有醋酸、酒精和福尔马林。它被广泛用于保存眼、骨髓、乳房、睾丸和其他组织以便组织学检查。
戴维森氏固定液davidson固定液
用作根治性切除标本中淋巴结可视化的过夜固定剂。通过将淋巴结转变为白色，有助于乳房和结肠标本检查。Moore和Barr使用了一种Hartman固定剂来固定皮肤活检标本，用于研究细核细节。使用常规和改良的Hartman固定剂将大鼠睾丸固定24小时以用于免疫组织化学和形态学比较，而将用于其他分析的眼睛和睾丸标本固定48小时。Description
Davidson's fixative (sometimes called Hartmann's fixative) is a rapid fixative. It is particularly useful for preparing tumors, bone marrow specimens, gynecologic material, fatty breast, and medical biopsies. It may be used for testicularbiopsies and for overnight fixation of fat containing lymph nodes. In addition, Davidson Solution is the preferred fixative for whole eyes, which provides optimal tissue preservation while avoiding retinal detachment.
Storage: RTStore in closed container (plastic or glass) to prevent evaporation.
Protocol
1. Use about 100 ml for bigger objects or several small specimens. Place specimen in the solution immediately after extraction. (A gauze pad should be used to keep floating objects submerged if necessary or inconsistent fixation will occur.)
2. Remain specimen in the solution for 1 -2 days. Important note: Fixation of small specimens is rapid. Exposure to the fixative should be limited to 24 hours (tissue may be transferred to neutral buffered formalin or 70% alcohol for storage).
3. The specimen then is taken out of the solution and placed either in formalin or 70% ethanol. Optional : To make very solid specimen (such as eyes) easier to cut, incubate in 70% ethanol for a day, transfer to 90% ethanol for a day and finally to 100% ethanol for a day.
戴维森氏固定液davidson固定液
Safety precautions:
1. Wear safety glasses, a lab coat, and gloves.
2. Prepare in area with adequate ventilation and away from heat or open flame.
davidson固定液产品简介：
Davidson's fixative是一种快速固定液，它具有良好的保存细胞核特点，并且产生的福尔马林色素最少，可用于常规组织病理学检查，Davidson's fixative固定液不含汞或其他金属，而且产生的收缩率更低，整体形态学细节也更好，因此被广泛用于保存眼、骨髓、乳房、睾丸和其他组织以便组织学检查。
davidson固定液操作步骤：
1. 将组织标本放入Davidson's fixative中，固定液的体积至少是标本体积的15～20倍。
2. 对于后续做免疫组化实验的组织固定标本不超过24小时，常规组织病理学实验的组织固定不超过48小时。
3. 从Davidson's fixative中取出组织标本，并用自来水短暂冲洗。
4. 织固定后暂时不用，可置于70%乙醇中保存。
davidson固定液注意事项：
1. Davidson's fixative有刺激性气味，请小心操作。
2. 组织取材的厚度不同，固定时间也不同。
3. 温度对固定的影响很明显，提高温度可以加速固定作用，但温度不宜过高。
4. 取出新鲜组织后，应及时固定，无法及时固定时，应保存于生理盐水中及时送检。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
davidson固定液，davidson固定液大量现货
