Nitsch培养基 植物
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Nitsch Medium
Nitsch培养基 植物
HYYZK021-50L
The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The plantlets matured and flowered profusely, but did not set seed.
Nitsch Medium含维生素 规格：50升
Total concentration Micro and Macro elements including vitamins: 2178.74 mg/l.
Nitsch Medium不含维生素 规格：50升
Total concentration Micro and Macro elements not including vitamins: 2070.19 mg/l.
Macro Elements
mg/l
mM
CaCl2
166.00
1.50
KH2PO4
68.00
0.50
KNO3
950.00
9.40
MgSO4
90.27
0.75
NH4NO3
720.00
9.00
Micro Elements
mg/l
μM
CuSO4.5H2O
0.025
0.10
FeNaEDTA
36.70
100.00
H3BO3
10.00
161.73
MnSO4.H2O
18.94
111.94
Na2MnO4.2H2O
0.25
1.03
ZnSO4.7H2O
10.00
34.78
Vitamins
mg/l
μM
Biotin
0.05
0.21
Folic acid
0.50
1.13
Glycine
2.00
26.64
myo-Inositol
100.00
554.94
Nicotinic acid
5.00
40.62
Pyridoxine HCl
0.50
2.43
Thiamine HCl
0.50
1.48
Nitsch J.P. and Nitsch C., Haploid plants from pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis in Nicotiana, Phytomorphology 19, 389 (1969).
