X-gluc

X-gluc

CAS:114162-64-0，hzk212-50mg  570元

产品简介

X-Gluc    GUS基因活性检测用底物

5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt

5-溴-4-氯-3-吲哚-β-D-葡萄糖苷酸酯

C14H13BrClNO7·C6H13N   MW 521.80  CAS:114162-64-0

GUS基因活性检测(GUS染色原理，X-gluc母液配制)

Appearance：Crystalline white powder

Purity > 98% by TLC and NMR

Storage: -20 ℃；protect from light and moisture.

Introduction：

The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the β-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized colour, making it useful in identifying GUS gene presence in most cell types and for the detection of the GUS gene fusion marker in plants.

X-Gluc has reported applications in the detection of contaminated food samples such as meat, dairy, and shellfish products.

X-Glucuronide also has clinical applications in the assessment of urinary tract infection by detecting the presence of E. coli. It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples by reducing false positives and negatives found with traditional methods.

Benefits：

● Saves time compared to other E. coli detection methods－24 hour direct plating method

● Minimizes errors－less than 1% false negatives and less than 5% false positives

● Easy visual detection－λmax is in visible range

References：

1. Janssen, Bart-Jan and R. C. Gardner, 1989. Localized transient expression of GUS in leaf discs following cocultivation with agrobacterium. Plant Molecular Biology 14:61-72.

2. Jefferson, R. A. 1987. Assaying chimeric genes in Plants: The GUS gene fusionsystem. Plant Molecular Biology Reporter 5:387-405.

3. Jefferson R. A., Kavanagh T. A., Bevan M. W. 1987. GUS fusions betaglucuronidase as a sensitive and versitle gene fusion marker in higher plants. EMBO J. 6:3901 3907.